ELISA Kit for Rat Pyruvate kinase PKM

Mfr.No.

E0588r

Alternative Names

Pyruvate kinase muscle isozyme ; Pkm2 ; Pykm

Categories

Elisa

Function

Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation.

Specificity

Natural and recombinant Rat Pyruvate kinase PKM

Subcellular Location

Cytoplasm Nucleus

Detection Range

1.25-80U/mL

Entryname

KPYM_RAT

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-105%	90-101%	95-105%	92-109%
EDTA plasma(n=5)	89-95%	93105%	89-94%	90-106%
heparin plasma(n=5)	93-104%	94-110%	101-111%	91-110%

Method Type

Sandwich

Platform

PF00224

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤3.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.7%

Protein Name

Pyruvate kinase PKM

Recovery

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	91-103
Plasma	98	93-106

Sensitivity

0.625U/mL

Sequence

50:

MPKPDSEAGT ; AFIQTQQLHA ; AMADTFLEHM ; CRLDIDSAPI ; TARNTGIICT ;

100:

IGPASRSVEM ; LKEMIKSGMN ; VARLNFSHGT ; HEYHAETIKN ; VRAATESFAS ;

150:

DPILYRPVAV ; ALDTKGPEIR ; TGLIKGSGTA ; EVELKKGATL ; KITLDNAYME ;

200:

KCDENILWLD ; YKNICKVVEV ; GSKIYVDDGL ; ISLQVKEKGA ; DYLVTEVENG ;

250:

GSLGSKKGVN ; LPGAAVDLPA ; VSEKDIQDLK ; FGVEQDVDMV ; FASFIRKAAD ;

300:

VHEVRKVLGE ; KGKNIKIISK ; IENHEGVRRF ; DEILEASDGI ; MVARGDLGIE ;

350:

IPAEKVFLAQ ; KMMIGRCNRA ; GKPVICATQM ; LESMIKKPRP ; TRAEGSDVAN ;

400:

AVLDGADCIM ; LSGETAKGDY ; PLEAVRMQHL ; IAREAEAAVF ; HRLLFEELAR ;

450:

ASSQSTDPLE ; AMAMGSVEAS ; YKCLAAALIV ; LTESGRSAHQ ; VARYRPRAPI ;

500:

IAVTRNPQTA ; RQAHLYRGIF ; PVLCKDAVLD ; AWAEDVDLRV ; NLAMNVGKAR ;

531:

GFFKKGDVVI ; VLTGWRPGSG ; FTNTMRVVPV ; P

Sequence Length

531

String

P11980

Sub Unit

Monomer and homotetramer. Exists as a monomer in the absence of FBP, and reversibly associates to form a homotetramer in the presence of FBP. The monomeric form binds T3. Tetramer formation induces pyruvate kinase activity. Interacts with HERC1 POU5F1 and PML.

Uniprot	P11980
Gene Name	Pkm
Immunogen Species	Rattus norvegicus (Rat)
Kegg	rno:25630
Unigene	Rn.1556

Reference	1. "The M1- and M2-type isozymes of rat pyruvate kinase are produced from the same gene by alternative RNA splicing." Noguchi T., Inoue H., Tanaka T. J. Biol. Chem.261:13807-13812(1986) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS M1 AND M2) 2. "Rat pyruvate kinase M gene: its complete structure and characterization of the 5'-flanking region." Takenaka M., Noguchi T., Inoue H., Yamada K., Matsuda T., Tanaka T. J. Biol. Chem.264:2363-2367(1989) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS M1 AND M2);SEQUENCE REVISION 3. "The nucleotide sequence of a full length cDNA encoding rat pituitary pyruvate kinase." Parkinson C., Kato H., Cheng S. Nucleic Acids Res.17:7106-7106(1989) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM M1) tissue: Pituitary. 4. Lubec G., Afjehi-Sadat L., Chen W.-Q., Kang S.U. Submitted (2007-07) to the UniProtKB [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE OF 33-56; 74-89; 93-115; 174-186; 208-246; 279-294; 343-376; 393-400; 423-436; 476-498 AND 505-526;PROTEIN SEQUENCE OF 384-399 (ISOFORM M2);IDENTIFICATION BY MASS SPECTROMETRY strain: Sprague-Dawley. tissue: Brain. tissue: Hippocampus. tissue: Spinal cord.

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ELISA Kit for Rat Pyruvate kinase PKM

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