ELISA Kit for Pig Erythropoietin

Mfr.No.

E0028p

Categories

Elisa

Function

Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass.

Specificity

Natural and recombinant Pig Erythropoietin

Subcellular Location

Secreted

Detection Range

0.156-10ng/mL

Entryname

EPO_PIG

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of EPO and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	96-100%	90-98%	95-102%	92-97%
EDTA plasma(n=5)	89-95%	93-98%	99-105%	90-97%
heparin plasma(n=5)	93-98%	99-105%	101-107%	96-105%

Method Type

Sandwich

Platform

PF00758

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.5%

Protein Name

Erythropoietin

Recovery

Recovery was determined by spiking various levels of EPO into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	96	94-99
Plasma	98	97-102

Sensitivity

0.063ng/mL

Sequence

50:

MGARECPARL ; LLLSLLLLPL ; GLPVLGAPPR ; LICDSRVLER ; YILEAKEGEN ;

100:

ATMGCAESCS ; FSENITVPDT ; KVNFYAWKRM ; EVQQQAMEVW ; QGLALLSEAI ;

150:

LQGQALLANS ; SQPSEALQLH ; VDKAVSGLRS ; LTSLLRALGA ; QKEAIPLPDA ;

194:

SPSSATPLRT ; FAVDTLCKLF ; RNYSNFLRGK ; LTLYTGEACR ; RRDR

Sequence Length

194

String

P49157

Uniprot	P49157
Gene Name	EPO
Immunogen Species	Sus Scrofa (Pig)
Kegg	ssc:397249
Unigene	Ssc.15896

Reference	1. "The porcine erythropoietin gene: cDNA sequence, genomic sequence and expression analyses in piglets." David R.B., Blom A.K., Sjaastad O.V., Harbitz I. Domest. Anim. Endocrinol.20:137-147(2001) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] strain: Norwegian Landrace. tissue: Kidney. 2. "Erythropoietin structure-function relationships: high degree of sequence homology among mammals." Wen D., Boissel J.-P.R., Tracy T.E., Gruninger R.H., Mulcahy L.S., Czelusniak J., Goodman M., Bunn H.F. Blood82:1507-1516(1993) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 5-194 tissue: Kidney.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13804406	E0028p	96T	$900		Add to Cart

ELISA Kit for Pig Erythropoietin

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