ELISA Kit for Pig Decorin

Mfr.No.

E6238p

Alternative Names

Bone proteoglycan II ; PG-S2

Categories

Elisa

Function

May affect the rate of fibrils formation.

Specificity

Natural and recombinant Pig Decorin

Subcellular Location

Secreted extracellular space extracellular matrix

Detection Range

78-5000pg/mL

Entryname

PGS2_PIG

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PGS2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	96-110%	98-107%	95-109%	99-108%
Saliva(n=3)	94-108%	93-109%	97-106%	95-107%
heparin plasma(n=5)	93-111%	95-105%	94-110%	98-106%

Method Type

Sandwich

Platform

PF00560

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤8.1%

Protein Name

Decorin

Recovery

Recovery was determined by spiking various levels of PGS2 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	103	94-105
Plasma	101	93-106

Sensitivity

35pg/mL

Sequence

50:

MKATIVFLLL ; AQVSWAGPFQ ; QKGLFDFMLE ; DEASGIGPED ; RFPEVPELEP ;

100:

LGPMCPFRCQ ; CHLRVVQCSD ; LGLDKVPKDL ; PPDTALLDLQ ; NNKITEIKDG ;

150:

DFKNLKNLHT ; LILINNKISK ; ISPGAFAPLV ; KLERLYLSKN ; QLKELPEKMP ;

200:

KTLQELRVHE ; NEITKVRKAV ; FNGLNQMIVV ; ELGTNPLKSS ; GIENGAFQGM ;

250:

KKLSYIRIAD ; TNITTIPQGL ; PPSLTELHLD ; GNKISKVDAA ; SLKGLNNLAK ;

300:

LGLGFNSIST ; VDNGSLANTP ; HLRELHLNNN ; KLNKVPGGLA ; EHKYIQVVYL ;

350:

HNNNISAVGS ; NDFCPPGYNT ; KKASYSGVSL ; FSNPVQYWEI ; QPSTFRCVYV ;

360:

RSAIQLGNYK ;

Sequence Length

360

String

Q9XSD9

Sub Unit

Binds to type I and type II collagen, fibronectin and TGF-beta. Forms a ternary complex with MFAP2 and ELN. Interacts with DPT.

Uniprot	Q9XSD9
Gene Name	DCN
Immunogen Species	Sus Scrofa (Pig)
Kegg	ssc:396957
Unigene	Ssc.19012

Reference	1. "Cloning of the porcine decorin gene." Stephenson S., Schnoke M., Vesely I. Submitted (1999-02) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG) strain: Yorkshire. 2. "Alternatively spliced version of the porcine decorin gene." Stephenson S., Schnoke M., Vesely I. Submitted (1999-04) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT) strain: Yorkshire. tissue: Aorta.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13809332	E6238p	96T	$900		Add to Cart

ELISA Kit for Pig Decorin

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