ELISA Kit for Mouse Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32

Mfr.No.

E14813m

Alternative Names

DEAH box protein 32 ; MuDDX32 ; Ddx32

Categories

Elisa

Specificity

Natural and recombinant Mouse Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32

Subcellular Location

Nucleus Mitochondrion

Detection Range

0.78-50ng/mL

Entryname

DHX32_MOUSE

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	95-104%	93-101%	96-104%	92-106%
EDTA plasma(n=5)	87-96%	91-103%	90-96%	88-103%
heparin plasma(n=5)	93-103%	92-105%	99-109%	89-108%

Method Type

Sandwich

Platform

PF04408

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤ 6.2%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤ 8.9%

Protein Name

Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32

Recovery

Recovery was determined by spiking various levels of ACE into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	97	89-102
Plasma	98	92-105

Sensitivity

0.439ng/mL

Sequence

50:

MDEEELDHPN ; ASPEKRYFPE ; SLDSSDGDEE ; GVLACEDLEL ; NPFDGLPYSS ;

100:

RYYKLLKERE ; ELPIWKEKYS ; FMESLLQNQV ; VVVSGDSKCG ; KSSQVPQWCA ;

150:

EYCLSIHYQH ; GGVICTQAHK ; QTAVQLALRV ; ADEMDVNIGH ; EVGYVIPFEN ;

200:

CCTTETILRY ; CTDDMLQREM ; MSNPFLGSYG ; VIILDDVHER ; SLATDVLLGL ;

250:

LKDVLLARPE ; LKLIVNCSPL ; LTSKLSSYYG ; DVPVIEVRNK ; HPVEVVYLSG ;

300:

AQKDSFESVI ; RLIFEIHRSG ; EKGDVVVFLA ; CEQDIEKTYE ; LVCQEGSNLN ;

350:

PDVGDLVVIP ; LYPKEKCSLF ; RPVDETEKRC ; QVYQRRVVLT ; TSCGESLIWS ;

400:

HTVKFVIDVG ; LERRQVYNPR ; IRANSLVLQP ; ISQSQAEIRK ; QLLGSSPSGK ;

450:

LFCLYTEEFA ; SKDMRPLKPA ; EMQEANLTSM ; VLFMKRVDIA ; GLGRCDFMNR ;

500:

PAPESLMQAL ; EDLDYLAALD ; NDGNLSEFGI ; IMSEFPLDPQ ; LSKSILASCE ;

550:

FDCVDEMLTI ; AAMVTAPSCF ; LHVPHGAEEA ; AVTCWKTFLH ; PEGDHFTLIN ;

600:

VYNAYQDTVL ; NSANEHCVEM ; WCHDCFLSCS ; ALRMADVIRA ; ELLEIIKRIE ;

650:

LPYAEPAFGS ; KENGLNIKKA ; LLSGYFMQIA ; RDVDGSGNYL ; MLTHKQVAQL ;

700:

HPLSSYSITK ; KMPEWVLFHQ ; FSISENNYIR ; VASAVSPELF ; MQLVPQYYFS ;

744:

NLPPSESKDI ; LQQAAGHLPT ; ETVNKDQDVC ; DKCPDATEQR ; CTIQ

Sequence Length

744

Uniprot	Q8BZS9
Gene Name	Dhx32
Immunogen Species	Mus musculus (Mouse)
Kegg	mmu:101437
Unigene	Mm.199223

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SKU	Supplier No.	Size	Your price	Quantity
BHE13803659	E14813m	96T	$776		Add to Cart

ELISA Kit for Mouse Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32

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