ELISA Kit for Mouse Hyaluronidase-4

Mfr.No.

E10858m

Alternative Names

Chondroitin sulfate endo-beta-N-acetylgalactosaminidase ; Chondroitin sulfate hydrolase ; CSHY ; Hyaluronoglucosaminidase-4

Categories

Elisa

Function

Endo-hyaluronidase that degrades hyaluronan to smaller oligosaccharide fragments. Has also chondroitase activity.

Specificity

Natural and recombinant Mouse Hyaluronidase-4

Subcellular Location

Membrane Multi-pass membrane protein

Detection Range

0.156-10ng/mL

Entryname

HYAL4_MOUSE

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HYAL4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	88-100%	90-99%	87-103%	92-98%
Saliva(n=3)	89-103%	93-98%	89-101%	90-103%
heparin plasma(n=5)	93-100%	94-99%	88-105%	91-102%

Method Type

Sandwich

Platform

PF01630

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤6.7%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤10.5%

Protein Name

Hyaluronidase-4

Recovery

Recovery was determined by spiking various levels of HYAL4 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	94	90-101
Plasma	96	93-106

Sensitivity

0.085ng/mL

Sequence

50:

MQLLPEGQLR ; LCVFQPVHLT ; SGLLILFILK ; SISSLKPARL ; PVYQRKPFIA ;

100:

AWNAPTDLCL ; IKYNLTLNLK ; VFQMVGSPRL ; KDRGQNVVIF ; YANRLGYYPW ;

150:

YTSEGVPING ; GLPQNTSLQV ; HLKKAAQDIN ; YYIPSENFSG ; LAVIDWEYWR ;

200:

PQWARNWNTK ; DIYRQKSRTL ; ISDMKENISA ; ADIEYSAKAT ; FEKSAKAFME ;

250:

ETIKLGSKSR ; PKGLWGYYLY ; PDCHNYNVYA ; TNYTGSCPEE ; EVLRNNDLSW ;

300:

LWNSSTALYP ; AVSIRKSFAD ; SENTLHFSRF ; RVRESLRIST ; MTSQDYALPV ;

350:

FVYTQLGYKE ; EPLLFLSKQD ; LISTIGESAA ; LGAAGIVVWG ; DMNLTSSEEN ;

400:

CTKVNRFVNS ; DFGSYIINVT ; RAAEVCSRHL ; CKNNGRCVRK ; TWKAAHYLHL ;

450:

NPASYHIEAS ; EDGEFIVRGR ; ASDTDLAVMA ; ENFLCHCYEG ; YEGADCREMT ;

481:

EASGPSGLSL ; SSSSVITLCL ; LVLAGYQSIQ ; L

Sequence Length

481

String

Q05A56

Uniprot	Q05A56
Gene Name	Hyal4
Immunogen Species	Mus musculus (Mouse)
Kegg	mmu:77042
Unigene	Mm.66017

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SKU	Supplier No.	Size	Your price	Quantity
BHE13805399	E10858m	96T	$776		Add to Cart

ELISA Kit for Mouse Hyaluronidase-4

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