ELISA Kit for Chicken Protein disulfide-isomerase

Mfr.No.

E1061c

Alternative Names

Cellular thyroid hormone-binding protein ; Prolyl 4-hydroxylase subunit beta ; Retina cognin ; R-cognin ; PDI ; PDIA1

Categories

Elisa

Function

This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase.

Specificity

Natural and recombinant Chicken Protein disulfide-isomerase

Subcellular Location

Endoplasmic reticulum lumen Cell membrane Peripheral membrane protein Highly abundant. In some cell types, seems to be also secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources. Localizes near CD4-enriched regions on lymphoid cell surfaces.

Entryname

PDIA1_CHICK

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PDIA1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-100%	91-103%	89-104%	91-99%
EDTA plasma(n=5)	93-99%	90-104%	92-99%	93-98%
heparin plasma(n=5)	95-98%	92-101%	90-100%	94-100%

Platform

PF00085

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤5.8%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤9.7%

Protein Name

Protein disulfide-isomerase

Recovery

Recovery was determined by spiking various levels of PDIA1 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	96	91-99
Plasma	97	95-102

Sequence

50:

MAVVRVRAIV ; ALLCLVAALG ; LAEPLEEEDG ; VLVLRAANFE ; QALAAHRHLL ;

100:

VEFYAPWCGH ; CKALAPEYAK ; AAAQLKAEGS ; EIRLAKVDAT ; EEAELAQQFG ;

150:

VRGYPTIKFF ; RNGDKAAPRE ; YTAGREADDI ; VSWLKKRTGP ; AATTLTDAAA ;

200:

AETLVDSSEV ; VVIGFFKDVT ; SDAAKEFLLA ; AESVDDIPFG ; ISSSADVFSK ;

250:

YQLSQDGVVL ; FKKFDEGRNN ; FEGDLTKDNL ; LNFIKSNQLP ; LVIEFTEQTA ;

300:

PKIFGGEIKT ; HILLFLPKSV ; SDYEGKLDNF ; KTAAGNFKGK ; ILFIFIDSDH ;

350:

SDNQRILEFF ; GLKKEECPAV ; RLITLEEEMT ; KYKPESDDLT ; ADKIKEFCNK ;

400:

FLEGKIKPHL ; MSQDLPEDWD ; KQPVKVLVGK ; NFEEVAFDEN ; KNVFVEFYAP ;

450:

WCGHCKQLAP ; IWDKLGETYR ; DHENIVIAKM ; DSTANEVEAV ; KIHSFPTLKF ;

500:

FPAGSGRNVI ; DYNGERTLEG ; FKKFLESGGQ ; DGAAADDDLE ; DLETDEETDL ;

515:

EEGDDDEQKI ; QKDEL

Sequence Length

515

String

P09102

Sub Unit

Homodimer. Monomers and homotetramers may also occur. Also constitutes the structural subunit of prolyl 4-hydroxylase. Stabilizes this enzyme and retains it in the ER without contributing to the catalytic activity. Binds UBQLN1.

Uniprot	P09102
Gene Name	P4HB
Immunogen Species	Gallus Gallus (Chicken)
Kegg	gga:374091
Unigene	Gga.4976

Reference	1. Hausman R.E. Submitted (2001-06) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA];SEQUENCE REVISION TO 315; 356; 488; 491-493 AND 497 2. "cDNA for R-cognin: homology with a multifunctional protein." Krishna Rao A.S., Hausman R.E. Proc. Natl. Acad. Sci. U.S.A.90:2950-2954(1993) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 139-515 tissue: Retina. 3. "Structure of the gene encoding the beta-subunit of chicken prolyl 4-hydroxylase." Nakazawa M., Aida T., Everson W.V., Gonda M.A., Hughes S.H., Kao W.W. Gene71:451-460(1988) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE OF 1-129; 163-172; 209-218; 244-253; 286-295; 353-463 AND 483-515 4. "Molecular cloning of a multifunctional chicken protein acting as the prolyl 4-hydroxylase beta-subunit, protein disulphide-isomerase and a cellular thyroid-hormone-binding protein. Comparison of cDNA-deduced amino acid sequences with those in other species." Parkkonen T., Kivirikko K.Z., Pihlajaniemi T. Biochem. J.256:1005-1011(1988) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 26-515;PROTEIN SEQUENCE OF 23-31 AND 95-109 5. Bassuk J.A. Submitted (1988-02) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 399-459

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ELISA Kit for Chicken Protein disulfide-isomerase

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