ELISA Kit for Chicken Pepsin A

Mfr.No.

E0632c

Categories

Elisa

Function

Shows particularly broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent.

Specificity

Natural and recombinant Chicken Pepsin A

Entryname

PEPA_CHICK

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Pepsin A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	89-98%	78-92%	86-98%	100-106%
EDTA plasma(n=5)	83-92%	86-99%	91-104%	87-98%
heparin plasma(n=5)	86-101%	89-97%	93-105%	90-102%

Platform

PF07966

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.4%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.3%

Protein Name

Pepsin A

Recovery

Recovery was determined by spiking various levels of Pepsin A into serum and plasma .

Sample Type	Average(%)	Recovery Range (%)
Serum	97	94-99
Plasma	96	92-102

Sequence

50:

SIHRVPLKKG ; KSLRKQLKDH ; GLLEDFLKKH ; PYNPASKYHP ; VLTATESYEP ;

100:

MTNYMDASYY ; GTISIGTPQQ ; DFSVIFDTGS ; SNLWVPSIYC ; KSSACSNHKR ;

150:

FDPSKSSTYV ; STNETVYIAY ; GTGSMSGILG ; YDTVAVSSID ; VQNQIFGLSE ;

200:

TEPGSFFYYC ; NFDGILGLAF ; PSISSSGATP ; VFDNMMSQHL ; VAQDLFSVYL ;

250:

SKDGETGSFV ; LFGGIDPNYT ; TKGIYWVPLS ; AETYWQITMD ; RVTVGNKYVA ;

300:

CFFTCQAIVD ; TGTSLLVMPQ ; GAYNRIIKDL ; GVSSDGEISC ; DDISKLPDVT ;

350:

FHINGHAFTL ; PASAYVLNED ; GSCMLGFENM ; GTPTELGEQW ; ILGDVFIREY ;

367:

YVIFDRANNK ; VGLSPLS

Sequence Length

367

Uniprot	P00793
Gene Name	PGA
Immunogen Species	Gallus Gallus (Chicken)
Unigene	Gga.402

Reference	1. "Covalent structure of chicken pepsinogen." Baudys M., Kostka V. Eur. J. Biochem.136:89-99(1983) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PROTEIN SEQUENCE

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SKU	Supplier No.	Size	Your price	Quantity
BHE13808996	E0632c	96T	$900		Add to Cart

ELISA Kit for Chicken Pepsin A

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