ELISA Kit for Bovine Melanocyte protein PMEL

Mfr.No.

E2299b

Alternative Names

Melanocyte protein Pmel 17 ; Premelanosome protein ; Retinal pigment epithelial-specific protein ; Silver locus protein homolog ; PMEL17 ; RPE1 ; SILV

Categories

Elisa

Function

Plays a central role in the biogenesis of melanosomes. Involved in the maturation of melanosomes from stage I to II. The transition from stage I melanosomes to stage II melanosomes involves an elongation of the vesicle, and the appearance within of distinct fibrillar structures.

Specificity

Natural and recombinant Bovine Melanocyte protein PMEL

Subcellular Location

M-alpha Secreted

Entryname

PMEL_BOVIN

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PMEL and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	104-110%	102-113%	100-116%	104-114%
EDTA plasma(n=5)	105-110%	98-116%	104-112%	102-112%
heparin plasma(n=5)	100-114%	103-112%	100-115%	101-113%

Platform

PF00801

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤7.9%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤11.1%

Protein Name

Melanocyte protein PMEL

Recovery

Recovery was determined by spiking various levels of PMEL into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	108	105-113
Plasma	107	104-112

Sequence

50:

MDLVLRKYLL ; HVALMGVLLA ; VGTTEGPRDR ; DWLGVSRQLR ; IKAWNRQLYP ;

100:

EWTESQGPDC ; WRGGHISLKV ; SNDGPTLIGA ; NASFSIALHF ; PKSQKVLPDG ;

150:

QVIWANNTII ; NGSQVWGGQL ; VYPQEPDDTC ; IFPDGEPCPS ; GPLSQKRCFV ;

200:

YVWKTWDQYW ; QVLGGPVSGL ; SIGTDKAMLG ; TYNMEVTVYH ; RRGSQSYVPL ;

250:

AHSSSAFTIT ; DQVPFSVSVS ; QLQALDGRNK ; RFLRKQPLTF ; ALQLHDPSGY ;

300:

LAGADLSYTW ; DFGDSTGTLI ; SRALTVTHTY ; LESGPVTAQV ; VLQAAIPLTS ;

350:

CGSSPVPGTT ; DRHVTTAEAP ; GTTAGQVPTT ; EVMGTTPGQV ; PTAEAPGTTV ;

400:

GWVPTTEDVG ; TTPEQVATSK ; VLSTTPVEMP ; TAKATGRTPE ; VSTREPSGTT ;

450:

VTQGTTPELV ; ETTAGEVSTP ; EPAGSNTSSF ; MPTEGTAGSL ; SPLPDDTATL ;

500:

VLEKRQAPLD ; CVLYRYGSFS ; LTLDIVQGIE ; SAEILQAVSS ; SEGDAFELTV ;

550:

SCQGGLPKEA ; CMDISSPGCQ ; LPAQRLCQPV ; PPSPACQLVL ; HQVLKGGSGT ;

600:

YCLNVSLADA ; NSLAMVSTQL ; VMPGQEAGLR ; QAPLFVGILL ; VLTALLLASL ;

649:

IYRRRLMKQG ; SAVPLPQLPH ; GRTQWLRLPW ; VFRSCPIGES ; KPLLSGQQV

Sequence Length

649

String

Q06154

Sub Unit

Heterooligomer; disulfide-linked heterooligomers of M-alpha and M-beta. Interacts with MLANA.

Uniprot	Q06154
Gene Name	PMEL
Immunogen Species	Bos Taurus (Cattle)
Kegg	bta:281487
Unigene	Bt.5091

Reference	1. "Investigation on the genetic background of coat color dilution in a Charolais x German Holstein F2 resource population." Kuehn C., Weikard R. Submitted (2006-10) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] tissue: Skin. 2. "The cDNA RPE1 and monoclonal antibody HMB-50 define gene products preferentially expressed in retinal pigment epithelium." Kim R.Y., Wistow G.J. Exp. Eye Res.55:657-662(1992) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 158-649 tissue: Retina.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13808910	E2299b	96T	$900		Add to Cart

ELISA Kit for Bovine Melanocyte protein PMEL

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