ELISA Kit for Bovine Lipase maturation factor 1

Mfr.No.

E10818b

Alternative Names

Transmembrane protein 112 ; TMEM112

Categories

Elisa

Function

Involved in the maturation of specific proteins in the endoplasmic reticulum. Required for maturation and transport of active lipoprotein lipase (LPL) through the secretory pathway.

Specificity

Natural and recombinant Bovine Lipase maturation factor 1

Subcellular Location

Endoplasmic reticulum membrane Multi-pass membrane protein

Entryname

LMF1_BOVIN

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of LMF1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	93-105%	92-108%	95-102%	90-106%
Saliva(n=3)	90-106%	93-105%	92-106%	93-101%
heparin plasma(n=5)	91-103%	94-102%	95-101%	91-106%

Platform

PF06762

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤7.1%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤6.5%

Protein Name

Lipase maturation factor 1

Recovery

Recovery was determined by spiking various levels of LMF1 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	99	92-103
Plasma	98	94-106

Sequence

50:

MAAPRESLRR ; RKAGAGDPEP ; EAPPGQGRDL ; KGRPARLRAG ; TFWLTRIVLL ;

100:

RALAFVYFVA ; FLVAFHQNKQ ; LIGDRGLLPC ; RAYLQSVQRH ; FGGRVSWDAL ;

150:

SYAPTILWLL ; DWSHMDANLD ; ALALLGLGIS ; SFILVSGCAN ; MVLMAALWVL ;

200:

YMSLVNVGQI ; WYSFGWESQL ; LETGFLGIFL ; CPLWTLSALP ; RGTPTSWVVM ;

250:

WGFRWLIFRI ; MLGAGLIKIR ; GDRCWRDLTC ; MDFHYETQPV ; PNPVAYFLHR ;

300:

SPWWFHRFET ; LSNHFLELVV ; PFFIFLGRRM ; CIVHGALQVL ; FQVVLIISGN ;

350:

LSFLNWLTIV ; PSLACFDDAT ; LGGLFPSGPG ; RLKDQVLKIQ ; EEETRGARAP ;

400:

RTRGSVARGT ; VNLALGILVA ; WLSIPVVLNL ; LSPRQVMNSS ; FNPLRIVNTY ;

450:

GAFGSITRER ; TEVILQGTAS ; ANASAPDSAW ; EDYEFKCKPG ; DPRRRPCLIS ;

500:

PYHHRLDWLM ; WFAAFQTYEH ; NEWIIHLAGK ; LLANDAQALS ; LLARNPFEGR ;

550:

DPPRWVRGEH ; YRYKFSRPGG ; RHAAEGKWWI ; RRRLGPYFPP ; LSRQDLRGYF ;

561:

TSRQWPYPEP ; E

Sequence Length

561

Uniprot	Q0P5C0
Gene Name	LMF1
Immunogen Species	Bos Taurus (Cattle)
Kegg	bta:505124
Unigene	Bt.44828

Reference	1. NIH - Mammalian Gene Collection (MGC) project Submitted (2006-08) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] strain: Hereford. tissue: Fetal pons.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13806556	E10818b	96T	$900		Add to Cart

ELISA Kit for Bovine Lipase maturation factor 1

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