ELISA Kit for Bovine C-C motif chemokine 2

Mfr.No.

E0087b

Alternative Names

Acidic seminal fluid protein ; Monocyte chemotactic protein 1A ; MCP-1 ; Small-inducible cytokine A2 ; SCYA2

Categories

Elisa

Function

Chemotactic factor that attracts monocytes, but not neutrophils.

Specificity

Natural and recombinant Bovine C-C motif chemokine 2

Detection Range

31.2-2000pg/mL

Entryname

CCL2_BOVIN

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CCL2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	92-98%	96-103%	97-105%	102-108%
EDTA plasma(n=5)	96-101%	98-105%	99-106%	91-96%
heparin plasma(n=5)	90-103%	91-104%	94-105%	95-102%

Method Type

Sandwich

Platform

PF00048

Precision

Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.5%

Protein Name

C-C motif chemokine 2

Recovery

Recovery was determined by spiking various levels of CCL2 into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	97	91-99
Plasma	96	94-103

Sensitivity

13.3pg/mL

Sequence

50:

MKVSAALLCL ; LLTVAAFSTE ; VLAQPDAINS ; QVACCYTFNS ; KKISMQRLMN ;

99:

YRRVTSSKCP ; KEAVIFKTIL ; GKELCADPKQ ; KWVQDSINYL ; NKKNQTPKP

Sequence Length

String

P28291

Uniprot	P28291
Gene Name	CCL2
Immunogen Species	Bos Taurus (Cattle)
Kegg	bta:281043
Unigene	Bt.2408

Reference	1. "Gene expression and cDNA cloning identified a major basic protein constituent of bovine seminal plasma as bovine monocyte-chemoattractant protein-1 (MCP-1)." Wempe F., Henschen A., Scheit K.H. DNA Cell Biol.10:671-679(1991) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] tissue: Seminal plasma. 2. "Characterization by cDNA cloning of the mRNA of a new growth factor from bovine seminal plasma: acidic seminal fluid protein." Wempe F., Einspanier R., Scheit K.H. Biochem. Biophys. Res. Commun.183:232-237(1992) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] tissue: Seminal plasma. 3. "Characterization of the bovine monocyte chemoattractant protein-1 gene." Wempe F., Kuhlmann J.K., Scheit K.H. Biochem. Biophys. Res. Commun.202:1272-1279(1994) [PubMed] [Europe PMC] [Abstract] [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] 4. "U.S. veterinary immune reagent network: expressed bovine gene sequences." U.S. Veterinary Immune Reagent Network, Hudgens T., Tompkins D., Baldwin C.L. Submitted (2007-11) to the EMBL/GenBank/DDBJ databases [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] strain: Belted Galloway. tissue: Peripheral blood.

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BHE13802095	E0087b	96T	$900		Add to Cart

ELISA Kit for Bovine C-C motif chemokine 2

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