gamma-GT is a key enzyme in the gamma-glutamyl cycle and catalyzes the degradation of GSH. gamma-GT catalyzes the transfer of the gamma-glutamyl group on GSH or other gamma-glutamyl compounds to the receptor, and can also catalyze the hydrolysis of GSH and other gamma-glutamyl compounds to produce glutamate, and is outside the cell Glutathione plays an important role in the metabolism.
Description
Abbkine CheKine Gamma-Glutamyl Transpeptidase (GGT) Activity Colorimetric Assay Kit is designed for detecting gamma-GT enzyme activity in the sample.
Featured
Determination of gamma-GT enzyme activity in a variety of biological samples such as tissues, cell,serum, bacterial. Detailed sample preparation and result calculation methods are provided. Simple operation steps.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Tag
GGT
Kit Components
Extraction Buffer Reagent I Reagent II Reagent III
Notes
The kit provides a simple method for detecting gamma-GT enzyme activity in a variety of biological samples such as tissues, cell,serum, bacterial. In the assay, gamma-GT catalyzes the transfer of gamma-glutamyl group in glutamyl p-nitroaniline to N-glycylglycine to generate p-nitroaniline, which has characteristic light absorption at 405nm; Calculate gamma-GT enzyme activity by measuring the increase rate of light absorption at 405nm.
Usage Notes
Do not mix or substitute reagents or materials from other kit lots or vendors. Avoid foaming or bubbles when mixing or reconstituting components. Avoid cross-contamination, change pipette tips between additions of standards, samples and reagents. Also, use separate reservoirs for each reagent. Ensure all reagents and equipment are at the appropriate temperature before starting the assay.
Storage Instructions
Storage at 4°C and protected from light upon receipt. Kit has a storage time of 6 months form receipt.
Applications
Cell Metabolism
Reviews of CheKine Micro Gamma-Glutamyl Transpeptidase (GGT) Activity Assay Kit