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Home / Antibodies

Tri-Methyl-Histone H3 (Lys14)/H3K14me3 Antibody

SKU : BHA12904973

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AFFINITY BIOSCIENCES LTD

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Mfr.No.	DF7253
Alternative Names	; H3.3A ; HIST1 cluster, H3E ; H3 histone family, member A ; H3.1 ; H3/l ; H3F3 ; H3FF ; H3FJ ; H3FL ; Histone gene cluster 1, H3 histone family, member E ; histone H3.1t ; Histone H3/o ; FLJ92264 ; H 3 ; H3 ; H3 histone family, member B ; H3 histone family, member C ; H3 histone family, member D ; H3 histone family, member F ; H3 histone family, member H ; H3 histone family, member I ; H3 histone family, member J ; H3 histone family, member K ; H3 histone family, member L ; H3 histone family, member T ; H3 histone, family 3A ; H3/A ; H3/b ; H3/c ; H3/d ; h3/f ; H3/h ; H3/i ; H3/j ; H3/k ; H3/t ; H31_HUMAN ; H3F1K ; H3F3A ; H3FA ; H3FB ; H3FC ; H3FD ; H3FH ; H3FI ; H3FK ; HIST1 cluster, H3A ; HIST1 cluster, H3B ; HIST1 cluster, H3C ; HIST1 cluster, H3D ; HIST1 cluster, H3F ; HIST1 cluster, H3G ; HIST1 cluster, H3H ; HIST1 cluster, H3I ; HIST1 cluster, H3J ; HIST1H3A ; HIST1H3B ; HIST1H3C ; HIST1H3D ; HIST1H3E ; HIST1H3F ; HIST1H3G ; HIST1H3H ; HIST1H3I ; HIST1H3J ; HIST3H3 ; Histone 1, H3a ; Histone 1, H3b ; Histone 1, H3c ; Histone 1, H3d ; Histone 1, H3e ; Histone 1, H3f ; Histone 1, H3g ; Histone 1, H3h ; Histone 1, H3i ; Histone 3, H3 ; histone cluster 1 H3 family member a ; histone cluster 1 H3 family member b ; histone cluster 1 H3 family member c ; histone cluster 1 H3 family member d ; histone cluster 1 H3 family member e ; histone cluster 1 H3 family member f ; histone cluster 1 H3 family member g ; histone cluster 1 H3 family member h ; histone cluster 1 H3 family member i ; histone cluster 1 H3 family member j ; Histone cluster 1, H3a ; Histone cluster 1, H3b ; Histone cluster 1, H3c ; Histone cluster 1, H3d ; Histone cluster 1, H3e ; Histone cluster 1, H3f ; Histone cluster 1, H3g ; Histone cluster 1, H3i ; Histone cluster 1, H3j ; Histone gene cluster 1, H3 histone family, member A ; Histone gene cluster 1, H3 histone family, member B ; Histone gene cluster 1, H3 histone family, member C ; Histone gene cluster 1, H3 histone family, member D ; Histone gene cluster 1, H3 histone family, member F ; Histone gene cluster 1, H3 histone family, member G ; Histone gene cluster 1, H3 histone family, member H ; Histone gene cluster 1, H3 histone family, member I ; Histone gene cluster 1, H3 histone family, member J ; Histone gene cluster 1, H3A ; Histone gene cluster 1, H3B ; Histone gene cluster 1, H3C ; Histone gene cluster 1, H3D ; Histone gene cluster 1, H3E ; Histone gene cluster 1, H3F ; Histone gene cluster 1, H3G ; Histone gene cluster 1, H3H ; Histone gene cluster 1, H3I ; Histone gene cluster 1, H3J ; Histone H 3 ; Histone H3.1 ; Histone H3.2 ; Histone H3.3 ; Histone H3/a ; Histone H3/b ; Histone H3/c ; Histone H3/d ; Histone H3/f ; Histone H3/h ; Histone H3/i ; Histone H3/j ; Histone H3/k ; Histone H3/l ; Histone H3/m ;
Categories	Primary Antibodies
Clonality	polyclonal
Description	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Immunogen	A synthetic peptide of human H3K14me3.
Molecular Weight	Observed Mol.Wt.: 15kDa. Predicted Mol.Wt.: 16kDa.
Reactivity	Human, Mouse, Rat
Specificity	H3K14me3 Antibody detects endogenous levels of total H3K14me3.
Subcellular Location	Nucleus. Chromosome.

Uniprot

Q16695

Gene Id

8290

Sequence

10	20	30	40	50
MARTKQTARK	STGGKAPRKQ	LATKVARKSA	PATGGVKKPH	RYRPGTVALR
60	70	80	90	100
EIRRYQKSTE	LLIRKLPFQR	LMREIAQDFK	TDLRFQSSAV	MALQEACESY
110	120	130
LVGLFEDTNL	CVIHAKRVTI	MPKDIQLARR	IRGERA

Concentration	1mg/ml
Format	Liquid
Purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage Condition	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Applications	ELISA, WB
Dilution	WB1:500-1:2000 IHC1:50-1:200 IF1:20-1:50, ELISA(peptide) 1:20000-1:40000

Manual
MSDS
Sample Preparation For Western Blotting.
Western Blotting Handbook.

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SKU	Supplier No.	Size	Your price	Quantity
BHA12904973	DF7253	100ul	$280		Add to Cart
BHA12904973	DF7253	200ul	$350		Add to Cart

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