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Home / Antibodies

ATM (Ab-1981) Antibody

SKU : BHA10558998

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CUSABIO TECHONOLOGY LLC

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Mfr.No.	CSB-PA272996
Alternative Names	Ataxia telangiectasia mutated homolog; Ataxia telangiectasia mutated; kinase ATM
Categories	Primary Antibodies
Clonality	polyclonal
Description	ATM encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. Two transcript variants encoding different isoforms have been found for this gene.Gupta A. et al. (2005) Mol Cell Biol. 25(12): 5292-5305.Bernstein JL. et al. (2002) Breast Cancer Res. 4(6): 249-252.Silverman J. et al. (2004) Genes Dev. 18(17): 2108-2119.Nakada D. et al. (2003) Nucleic Acids Res. 31(6): 1715-1724.
Host	Rabbit
Immunogen	Peptide sequence around aa.1979~1983 (E-G-S-Q-S) derived from Human ATM.
Involvement In Disease	Ataxia telangiectasia (AT)
Raised In	Rabbit
Reactivity	Human, Mouse
Regulatory	RUO
Relevance	ATM encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. Two transcript variants encoding different isoforms have been found for this gene. Gupta A. et al. (2005) Mol Cell Biol. 25(12): 5292-5305. Bernstein JL. et al. (2002) Breast Cancer Res. 4(6): 249-252. Silverman J. et al. (2004) Genes Dev. 18(17): 2108-2119. Nakada D. et al. (2003) Nucleic Acids Res. 31(6): 1715-1724.
Species	Homo Sapiens (Human)
Specificity	The antibody detects endogenous level of total ATM protein.
Subcellular Location	Nucleus, Cytoplasmic vesicle

Uniprot	Q13315
Gene Id	472
Database Links	HGNC:795 OMIM:208900 KEGG:hsa:472 STRING:9606.ENSP00000278616 UniGene:Hs.367437
Function	Serine/threonine protein kinase which activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at double strand breaks (DSBs), thereby regulating DNA damage response mechanism. Also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. After the introduction of DNA breaks by the RAG complex on one immunoglobulin allele, acts by mediating a repositioning of the second allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. Also involved in signal transduction and cell cycle control. May function as a tumor suppressor. Necessary for activation of ABL1 and SAPK. Phosphorylates DYRK2, CHEK2, p53/TP53, FANCD2, NFKBIA, BRCA1, CTIP, nibrin (NBN), TERF1, RAD9 and DCLRE1C. May play a role in vesicle and/or protein transport. Could play a role in T-cell development, gonad and neurological function. Plays a role in replication-dependent histone mRNA degradation. Binds DNA ends. Phosphorylation of DYRK2 in nucleus in response to genotoxic stress prevents its MDM2-mediated ubiquitination and subsequent proteasome degradation. Phosphorylates ATF2 which stimulates its function in DNA damage response.
Pathway	p53 signaling pathway NF-kappa B signaling pathway Apoptosis Cell cycle Cellular senescence FoxO signaling pathway
Protein Families	PI3/PI4-kinase family, ATM subfamily
Tissue Specificity	Found in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, spleen, thymus, testis, ovary, small intestine, colon and leukocytes.
Weight	350,687 Da

Buffer	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form	Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format	liquid
Purification	Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Purity	Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Storage	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Applications

ELISA, WB, IF

Description

ELISA ; WB ; IF, WB ; ELISA ; IF

Dilution

WB	1:500-1:1000
IF	1:100-1:200

Tested Application

ELISA,WB,IF;WB:1:500-1:1000,IF:1:100-1:200

Datasheet
ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol

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SKU	Supplier No.	Size	Your price	Quantity
BHA10558998	CSB-PA272996	100ul	$297		Add to Cart

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