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Home / Primary Antibodies

AKT Antibody (pSer473) (R20271)

SKU : BHA17120085

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NSJ Bioreagents

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Mfr.No.	R20271-0.1ML
Clonality	Recombinant Rabbit Monoclonal
Description	This recombinant phospho-AKT antibody reacts to AKT1/2/3 only when phosphorylated at Ser473. There is no cross-reactivity with Akt without phosphorylation at Ser473. It may also react to bovine, mouse or rat phospho-AKT (Ser473), as predicted by immunogen homology
Formulation	Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Host	Rabbit
Immunogen Region	A phospho-peptide corresponding to human phospho-Akt (Ser473) was used as the immunogen for this recombinant phospho-AKT antibody.
Isotype	Rabbit IgG
Species Reactivity	Human
Note	The stated application concentrations are suggested starting points. Titration of the recombinant phospho-AKT antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.

Uniprot	P31749, P31751, Q9Y243

Format	Purified
Purity	Protein A purified from animal origin-free supernatant
Storage	Store the recombinant phospho-AKT antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).

Applications	WB, IHC
Description	Immunohistochemistry (FFPE): 1:200-1:500 (1),Western blot: 1:1000-1:2000

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHA17120085	R20271-0.1ML	100ul	$409		Add to Cart

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