rAAV-hSyn-FDIO-mGFP-T2A-Synaptophysin-mRuby

Mfr.No.	BC-0316
Promoter	hSyn
Fluorophore/tag	mGFP/Synaptophysin-mRuby
Tag Size	777/1632
Serotype	AAV1; AAV5; AAV8; AAV9; AAV.PHP.eB; AAV2; AAV6; AAV.rh10; AAV.DJ; AAV.DJ8; AAV.PHP.B; AAV.PHP.S; AAV2/RETRO; AAV.anc80
Description	This AAV expresses mGFP-T2A-Synaptophysin-mRuby driven by a neuron hSyn promoter in Flippase (FLP)-dependent manner. hSyn is a Human synapsin 1 gene promoter. The short 2A peptide sequences, when cloned in-frame between two genes, allow for efficient, stoichiometric production of discrete protein products within a single vector through a novel "cleavage" event within the 2A peptide sequence. This differs from conventional approaches for multiple protein expressions, such as IRES-mediated bicistronic gene expression, which has several limitations including imbalanced protein expression. The use of 2A peptide sequences alleviates these concerns, since 2A-mediated "self-cleavage" gives rise to a 1:1 ratio of the two separate proteins. Several forms of 2A peptide are commonly used: T2A (Thoseaasigna virus 2A), P2A (porcine teschovirus-1 2A), E2A (equine rhinitis A virus), and F2A (foot-and-mouth disease virus, FMDV 2A). mRuby is a basic (constitutively fluorescent) red fluorescent protein published in 2009, derived from Entacmaea quadricolor, with a Excitation/Emission spectrum around 558nm/605nm. It has low acid sensitivity. mGFP is a membrane-bound form of GFP containing a palmitoylation sequence of GAP43 at its N-terminus, with a Excitation/Emission spectrum around 488nm/507nm. FDIO is Flp-dependent Double-Floxed Inverted Open reading frame (fDIO) cassette.
Titer	2×10¹²vg/ml-5×10¹²vg/ml If you need a higher titer, please email us at [email protected]
Purification	Iodixanol gradient ultracentrifugation
Storage	Store at -80℃. Thaw just before use and keep on ice.

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Combination SKU	Supplier No.	Size	Serotype	Your price
BHV12401012-1	BC-0316	100ul	AAV2/1	$531	Add to Cart
BHV12401012-2	BC-0316	100ul	AAV2/2	$531	Add to Cart
BHV12401012-3	BC-0316	100ul	AAV2/5	$531	Add to Cart
BHV12401012-4	BC-0316	100ul	AAV2/6	$531	Add to Cart
BHV12401012-5	BC-0316	100ul	AAV2/6m	$531	Add to Cart
BHV12401012-6	BC-0316	100ul	AAV2/6.2FF	$531	Add to Cart
BHV12401012-7	BC-0316	100ul	AAV2/7m8	$531	Add to Cart
BHV12401012-8	BC-0316	100ul	AAV2/8	$531	Add to Cart
BHV12401012-9	BC-0316	100ul	AAV2/9	$531	Add to Cart
BHV12401012-10	BC-0316	100ul	AAV2/DJ	$531	Add to Cart
BHV12401012-11	BC-0316	100ul	AAV2/DJ8	$531	Add to Cart
BHV12401012-12	BC-0316	100ul	AAV2/Retro	$531	Add to Cart
BHV12401012-13	BC-0316	100ul	AAV2/PHP.eB	$531	Add to Cart
BHV12401012-14	BC-0316	100ul	AAV2/PHP.B	$531	Add to Cart
BHV12401012-15	BC-0316	100ul	AAV2/rh10	$531	Add to Cart
BHV12401012-16	BC-0316	100ul	AAV2/PHP.S	$531	Add to Cart
BHV12401012-17	BC-0316	100ul	AAV2/anc80	$531	Add to Cart
BHV12401012-18	BC-0316	100ul	AAV2/B10	$531	Add to Cart
BHV12401012-19	BC-0316	100ul	AAV2/olig001	$531	Add to Cart
BHV12401012-20	BC-0316	100ul	AAV2/BI30	$531	Add to Cart
BHV12401012-21	BC-0316	100ul	AAV2/BR1	$531	Add to Cart
BHV12401012-22	BC-0316	100ul	AAV2/MG1.2	$531	Add to Cart
BHV12401012-23	BC-0316	100ul	AAV1/2	$531	Add to Cart
BHV12401012-24	BC-0316	100ul	AAV2/MaCPNS1	$531	Add to Cart
BHV12401012-25	BC-0316	100ul	AAV2/MaCPNS2	$531	Add to Cart

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rAAV-hSyn-FDIO-mGFP-T2A-Synaptophysin-mRuby

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