SERVICES

Syno®-Synthetic DNA Libraries

Syno®-Synthetic DNA Libraries

Synbio Tech

Synbio Technologies is equipped with the world leading Syno® DNA technology platforms ranged from standard gene synthesis to the proprietary next generation Syno® 3.0 synthesis technology. We are capable of generating high quality DNA libraries of any size and complexity with an efficient timeframe and lower cost. Our synthetic DNA libraries can facilitate your various research interests: spanning from functional genetics to directed evolution, especially to protein engineering and antibody studies. In addition, Synbio Technologies’ experienced bioinformatics team can help to analyze and provide synthetic DNA libraries design to your researches.

Syno®3.0-Oligo Pool Synthesis

Syno®3.0-Oligo Pool Synthesis

Synbio Tech

Our innovative Syno® 3.0 process allows us to offer the highest quality DNA fragments,de novo synthesized genes, pathways, genomes and variant libraries at the lowest price in the industry. The breadth of our product portfolio runs from short linear ssDNA fragments to long, sequence-perfect, clonal genes and genomes in any vector of choice.

Syno®2.0-Gene Synthesis

Syno®2.0-Gene Synthesis

Synbio Tech

Syno® 2.0 platform of Synbio Tech. is an industrializedde novo DNA synthesis platform. In Syno® 2.0, our commitment is to translate the advanced DNA engineering technologies into reliable and cost-effective biological solutions. Our team has accumulated many advanced techniques that include large DNA fragments assembling (up to 150 Kb), manipulating toxic or unstable DNA fragments in a variety range of cell systems (for example: E. coli; Yeast; insect cell; mammalian cell, etc.). We offer services that include de novo gene/pathway/genome synthesis, DNA engineering, mutagenesis, DNA cloning and sub-cloning, PCR-cloning, plasmid DNA preparation, etc.. Our  innovative manufacturing process allows us to offer the highest quality DNA with the fastest turnaround time in the industry.

Syno®1.0-Oligo Synthesis

Syno®1.0-Oligo Synthesis

Synbio Tech

Syno® 1.0 platform of Synbio Tech. is an industrialized DNA chemical synthesis platform. Our innovative manufacturing process allows us to offer the highest quality oligos, labeled/modified oligos, degenerated oligo library, aptamers at the lowest price in the industry. The breadth of our service & product portfolio runs from short oligos to dsDNA fragments. 

Genetic Engineering Vaccine

Genetic Engineering Vaccine

Synbio Tech

By using synthetic biology technology, gene fragments of the protective antigen encoding of the pathogen are cloned into expression vector. After that, the expression vector is transformed or transfected into bacteria, yeast, or mammalian cells. Then, the vaccine is obtained after expression and purification. Genetic engineering vaccine is genetic deletion vaccine since virulence related genes of the pathogen have been removed.

Protein Directed Evolution

Protein Directed Evolution

Synbio Tech

In vitro directed evolution of proteins is an artificially simulated natural evolution mechanism. It creates mutant protein that does not exist in nature or one that some of its enzyme properties are significantly enhanced by using modern molecular biology methods to create a large number of gene variants library and adopting sensitive directed selection strategy. Directed evolution has been widely used in the molecular modification of proteins and is considered as the most efficient method for producing proteins with improved or completely new properties.

Humanized Antibody Library

Humanized Antibody Library

Synbio Tech

Traditional humanized antibody library mainly refers to a group of re-expressed antibody which is transformed by gene cloning and DNA recombination technology based on mouse monoclonal antibody. Most of its amino acid sequence was replaced by human source sequence. The affinity and specificity of the parental mouse monoclonal antibody are basically reserved, while its heterology is decreased, which is beneficial to be applied to human body.

Pathway design

Pathway design

Synbio Tech

Synthetic biology is a new interdisciplinary area of life science that refers to:

Metagenome Sequencing

Metagenome Sequencing

Synbio Tech

Metagenome is the study of the genome of the entire microbial community in a specific environmental sample. This is achieved by directly extracting the DNA of an environmental sample for high-throughput sequencing without isolation and culture. Metagenomic sequencing promotes research focusing on the macroscopic characteristics of microbial community structure, community diversity, species classification, functional activity, mutual cooperation and their relationship with the environment. Using this approach scientists can analyze the genetic composition and function of environmental micro-population, interpret the diversity and abundance of micro-populations, and explore the relationship between microorganism and environment, microorganism and hosts. It can more accurately reflect the actual living conditions of microorganisms.

Eukaryotic RNA sequencing

Eukaryotic RNA sequencing

Synbio Tech

Eukaryotic RNA sequencing is based on Next Generation Sequencing (NGS) and provides comprehensive and rapid access to all transcript sets of a particular cell or tissue in certain state. It is used to study gene structure and function, alternative splicing and prediction of new transcript, etc. Eukaryotic RNA sequencing can be divided into reference transcriptome and non-reference transcriptome according to with or without reference genomes.

Prokaryotic RNA sequencing

Prokaryotic RNA sequencing

Synbio Tech

Prokaryotic RNA sequencing is based on Next Generation Sequencing (NGS) platform to get all transcript information about prokaryotes at a certain time or environmental conditions by building a strand-specific RNA-seq library to sequence prokaryotic transcripts (mRNA and non-coding RNA). All sequence information of prokaryotes is obtained from gene sequence level and expression level in a specific situation. Through NGS, mRNA expression can be quantitatively analyzed, differentially express genes and their corresponding functional analysis, and also Non-coding RNAs (sRNAs) information can be figured out. Through the prediction of sRNAs target genes and the analysis of co-expression, the sRNAs corresponding function was clear, the molecular regulation mechanism and function of different phenotypes of microorganisms were revealed.

16S rDNA Sequencing

16S rDNA Sequencing

Synbio Tech

16S rDNA is a genetic sequence that encodes the 16S rRNA which is present all bacterial chromosomal genes. 16S rDNA is recognized by bacteriologists and taxonomists due to its moderate size of about 1.5 Kb. This size allows these scientists to determine the differences between different bacteria as well as easily determine the sequence through DNA sequencing. With these characteristics 16S rDNA sequencing can be used to detect a species’ classification, abundance, population structure, system evolution, and colony community of bacteria in environmental samples.

NGS High Throughput Sequencing

NGS High Throughput Sequencing

Synbio Tech

Synbio Technologies provides a wide range of second-generation sequencing services to comply with our customers’ specific requests. These services include capture chip preparation, library preparation, sample validation, as well as sequencing and bioinformatics analysis.

Long RNA Synthesis

Long RNA Synthesis

Synbio Tech

With the rapid development of the technologies related to gene editing, gene structure and function, the demand for long RNA synthesis has increased. One of the most common RNA synthesis requests is for long-stranded non-coding RNA (lncRNA), a non-coding RNA sequence with more than 200 nucleotides. These lncRNA play an important role in X-chromosome silencing, chromosome and genome modification, transcriptional activation, transcriptional interference, and intranuclear transport. The complex and precise regulatory functions of lncRNA in development and gene expression greatly contributes to the complexity of genome. lncRNA also offer researchers a fresh perspective to better understand the complexity of biological life from the dimension of gene expression regulatory network.

miRNA Synthesis

miRNA Synthesis

Synbio Tech

miRNAs are single stranded small non-coding RNA of 22 nucleotides that play important roles as endogenous gene regulators by mediating translation repression or promoting degradation of target mRNA. While the normal expression and function of miRNAs are vital for physiological processes, aberrant expression of miRNAs has been proved to be closely related to the occurrence of various cancers. Perturbation of endogeneous miRNAs helps to study the function of specific miRNAs and holds the potential for therapeutics.

Antibody Affinity Maturation Service

Antibody Affinity Maturation Service

Synbio Tech

The binding affinity of an antibody is one of the key parameter to determine the efficacy and dosage of an antibody drug. Currently, the methods to improve the binding affinity of antibodies mainly focus on site-mutation, CDR rearrangement, DNA recombinant, etc. With our unique and effective affinity maturation technology, we can quickly spot the key amino acid site in antibody domain. This, in turn, increases the binding affinity of the antibody, reaching at least nM level.

Antibody Humanization Service

Antibody Humanization Service

Synbio Tech

Immunogenicity or Human Anti-Mouse Antibodies (HAMA) response is what happens when antibodies derived from xenogeneic sources are administered to humans. This can potentially decrease the effectiveness of immune-treatment through rapid clearance of the therapeutic antibody or immune-conjugate. In some cases, the patient may even develop an allergic sensitivity and be at risk of anaphylactic shock upon any future treatment containing these “foreign” antibodies. Due to this potential risk of immunogenicity and HAMA response, the humanization of antibodies from xenogeneic sources to become safer and more reliable humanized antibody drugs is critical.

Single B Cell Antibody Service

Single B Cell Antibody Service

Synbio Tech

The immune system is a very robust “antibody factory” that consistently produces antibodies to fight infections and diseases by recognizing and targeting infected cells or pathogens (antigens). The emergence of single B cell antibody technology has enabled a new strategy for discovery of naturally occurring antibody drugs. The single B cell antibody technology produces higher throughput and higher coverage than the conventional hybridoma or phage display technologies. Most importantly, this technology also avoids the disadvantage of unnaturally paired heavy and light antibody chain displayed by phage.

Single Domain Antibody (sdAb) Service

Single Domain Antibody (sdAb) Service

Synbio Tech

Complex structures and high production costs associated with monoclonal antibodies (mAbs) have limited their achievable clinical applications. For this reason, scientists focusing on antibody drug discovery have shifted their interests toward recombinant antibody fragments.

Custom Monoclonal Antibody Services

Custom Monoclonal Antibody Services

Synbio Tech

Hybridoma technology features the effective usage of innate functions of both immune and cancer cells to generate monoclonal antibodies (mAbs). The resulting hybridoma cell lines are produced to combat specific antigens of interest. Such technology has forged remarkable new approaches toward therapeutic mAb discovery, as well as disease diagnosis and prevention.