Genetic Engineering Vaccine

Genetic Engineering Vaccine

Synbio Tech

By using synthetic biology technology, gene fragments of the protective antigen encoding of the pathogen are cloned into expression vector. After that, the expression vector is transformed or transfected into bacteria, yeast, or mammalian cells. Then, the vaccine is obtained after expression and purification. Genetic engineering vaccine is genetic deletion vaccine since virulence related genes of the pathogen have been removed.

sgRNA Bioinformatics Service

sgRNA Bioinformatics Service

Synbio Tech

The development of DNA sequencing and its large-scale application have promoted the development of precision and personalized medicine. DNA Sequencing helps to compare the mutated gene within different cells. However, there are often a number of features that obscure the direct relationship between genotype and disease phenotype. In recent years,CRISPR-Cas9 has been recognized as a popular and effective gene editing technology allowing scientists to alter the DNA sequence. This is done by precisely editing the living cell’s genome. Over time this modification will establish the foundation for the development of new therapies for human genetic diseases.

Yeast Genome Editing

Yeast Genome Editing

Synbio Tech

With the advancement of genome editing technology, more and more species can be genetically modified to satisfy a variety of requirements. Yeast is one of them. Yeasts are unicellular fungi and have been making contributions to human society from ancient times to the modern world. Apart from applications in baking and fermentation for thousands of years, yeast has also become an important model organism in contemporary cell biology and microbiology research. Recently, the CRISPR-Cas9 system has been used as an easy and cost-efficient method of genome modification in simple model organisms. Genome editing in yeast can help to produce efficient mutant yeast colonies and therefore promote their applications in different industries such as biopharmaceuticals, pollution control, energy, chemical bio-manufacturing, etc.

Complete Solution for Animal Gene Knock-out

Complete Solution for Animal Gene Knock-out

Synbio Tech

Animal cell experiments play a significant role in the life science research. Corresponding animal pathologic models can be constructed in order to observe the occurrence and development of diseases. However, it oftentimes takes several years to construct genetically modified animal models by traditional methods. Through CRISPR Cas9 technology, the time it takes to construct genetically modified animal models can be reduced to as little as a few weeks, greatly reducing the overall time and money spent doing these experiments.

CRISPR Cas9 Lentiviral Packaging and the Construction of Stable Cell Lines

CRISPR Cas9 Lentiviral Packaging and the Construction of Stable Cell Lines

Synbio Tech

Lentiviral vectors have a high transfection efficiency on mammalian cells and can provide an efficient way to integrate exogenous gene into host chromosome. As Cas9 protein has a longer ORF, conventional methods (electrotransfection or chemical reagents transfection) have a lower efficiency of inserting Cas9 gene into host cells. Hence, high-efficiency expression lentiviral vectors can be used to construct Cas9-expressing stable cell lines.

CRISPR Cas9 System Activity Detection

CRISPR Cas9 System Activity Detection

Synbio Tech

In the CRISPR-Cas9 system, different guide RNA (gRNA) can be designed based on the target gene. The DNA cutting efficiency of Cas9 can vary depending on the gRNA and target gene. It is therefore important to be able to accurately verify the activity of the CRISPR-Cas9 system in a given assay, in order to maximize the cleavage and gene knock-in/knock-out efficiency of CRISPR-Cas9. Synbio Technologies provides SSA activity assays,in vitro cleavage activity assays, and endogenous activity assays to detect gRNA activity.

CRISPR Cas9 sgRNA Panel

CRISPR Cas9 sgRNA Panel

Synbio Tech

Due to its simplicity and ease of customization, the CRISPR-Cas9 gene editing system is a popular tool in genetic screening. Recently, more and more literature report that CRISPR-Cas9 is more effective, reliable, and specific than RNAi in gene knockout experiments. Gene knockout screening utilizing CRISPR-Cas9 has been widely used for target and gene identification in many research areas, such as cancer development, drug resistance, immune response, bacterial toxin susceptibility, etc.

CRISPR Cas9 sgRNA Library Design and Screening

CRISPR Cas9 sgRNA Library Design and Screening

Synbio Tech

Studying gene functions in metabolic pathway or disease development is very important for disease mechanism research and drug discovery. Leading a new generation of gene editing technology, theCRISPR-Cas9 system is popular for its simplicity of operation and its universal applicability. Because of these unique advantages, CRISPR-Cas9 technology has become a powerful tool for forward genetic screening. Mutant libraries of various functions can be constructed by CRISPR, and the genes related to this function then identified through functional screening and enrichment, PCR amplification, and deep sequencing analysis.

CRISPR Cas9 sgRNA Vector Construction

CRISPR Cas9 sgRNA Vector Construction

Synbio Tech

The CRISPR-Cas9 system is one of the most popular types of gene editing technology, and has been used in widespread backgrounds over the last few years. Due to its ease of use and strong extensibility, CRISPR-Cas9 has been applied to fields including laboratory research, biomedicinal research, and genetic engineering of flora and fauna. This technology can also be applied to the construction of genetically modified animal models, construction of gene-regulated cell lines, breeding of animals and plants, repair of hereditary disease, cancer research, and treatments of other serious diseases/screening of relevant drug targets.

Ready-to-use CRISPR Cas9 sgRNA Synthesis

Ready-to-use CRISPR Cas9 sgRNA Synthesis

Synbio Tech

CRISPR-Cas9 is one of the most convenient types of gene editing technology, and has been widely used in editing genes of many species[1]. CRISPR-Cas9 generally involves construction a plasmid containing the specific endogenous promoter and connection of the Cas9 gene and a synthetic sgRNA, followed by transfer into the cell or animal. However, plasmid construction and verification are both tedious and time-consuming. The degradation of the plasmid is relatively slow, which may prove to be inconvenient for subsequent experiments.

ELISA Kits, Antibodies, Proteins, and Reagents for Life Scientists

ebiohippo is a top distributor of the best and most reliable ELISA and Assay kits. We can provide a wide range of kits from well-known suppliers and brands with authentic and validated data. We provide antibodies to help you quickly identify and quantify proteins, and evaluate other necessary data for various applications in life sciences.

Highly Validated Antibodies and Kits Available for Life Sciences Research

Our aim at ebiohippo is to facilitate and accelerate access to health for people everywhere. Through our life science products, we offer solutions for diagnostics and environmental testing as well. We aim to help scientists and engineers solve problems at every stage of their work. The provided lab materials, technologies, and services will help make research, and production, both pharmaceutical and biotech, simpler, faster, and safer. AT ebiohippo, we believe that we can enable scientific discovery by helping customers understand biological functions and diseases. ebiohippo works in partnership with the global scientific community to offer superior products and solutions, both for research, and for manufacturing of chemical and biological drugs.

ebiohippo aims to help you advance in life sciences with high-quality training and support. We can help your highly interdisciplinary bioscience research, from the molecular level, through the cellular and organism levels, as well as spans bacteria, viruses, fungi, animals, and plants. Live science research deals with a broad range of biology, biotechnology, genomics, proteomics, bioinformatics, pharmaceutical and biomedical research, and techniques. We provide you with the products that allow you to identify common principles that operate across the breadth of living systems and investigate solutions to major global challenges. Our products are used in research done with cutting-edge computational and experimental technologies in the life sciences research sector.

A Huge Variety of Elisa Kits from Different Sample Types

Our ELISA kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. All our kits are ready to use and we offer different formats to choose from including quantitative, semi-quantitative, indirect, or competitive. Along with that, our extensive catalog comprises of ELISA kits that can be used to study diverse biochemical pathways and diseases, such as cancer, the insulin pathway, and oxidative stress. Moreover, our kits are only assembled when you place an order to ensure that all of the components will be fresh and to extend the shelf life for our customers.

Assay Kits

ebiohippo offers thousands of Assay kits in multiple formats, including Enzyme activity, inhibitor screening, epigenetic, apoptosis, cell damage, cell proliferation and cytotoxicity, cell signaling, metabolism and other detections.

Easy to Use Kits, Versatile in Functionality and High in Compatibility

Our kits are easy to use yet robust for life science research and bioassays, and are optimized for use on our instruments. They can easily measure enzyme activity, monitor and signal pathway activity, detect and quantify cellular progress such as cell death, cell viability or metabolic reactions. The constituents of every kit are thoroughly tested for their compatibility with each other to give you accurate and authentic results.

Additionally, with our offered assay kits, you’ll be able to study changes in gene expression, and to screen small molecule inhibitors. At ebiohippo, we offer kits that allow researchers to quickly narrow down results by detection target, species reactivity, application or conjugate, and with them, you can obtain highly sensitive, detailed, and accurate results across a wide dynamic range with fast, efficient processing.

Primary Antibodies

At ebiohippo, we offer thousands of monoclonal/polyclonal antibodies and conjugates validated for use in a variety of common applications including WB, FC, IHC, ICC, IF, IP, and more. Our primary antibodies are developed as polyclonal or monoclonal, which use mouse, rat, rabbit, goat, and other animal species as hosts. The primary antibodies provided by us are produced and supplied in various forms, ranging from crude antiserum to antigen-purified preparations. We also offer you a superior antigen recognition system which is combined with the specificity and consistency of a monoclonal, bringing you the highest quality antibodies possible.

Secondary Antibodies

The secondary antibodies provided by ebiohippo perfectly bind to the primary antibody to help them assist in detection, sorting and purifying the target antigens. We offer secondary antibodies that work as staple tools in immunological applications for detecting, quantifying, or purifying target proteins and other molecules. We offer a diverse range of different target species and conjugates that allow a variety of possible combinations and provide flexibility in designing experiments. Along with that, the secondary antibodies we offer often conjugate to other molecules for detection, such as fluorophore, biotin, horseradish peroxidase, or alkaline phosphatase. We also provide you with a general guideline to help you in selecting the appropriate secondary antibody for your experiment.

Proteins and Peptide

With the diverse protein and peptide sources available at ebiohippo, we believe we can fulfill biochemical research needs with ease. The biomolecules we provide can fulfill a diverse range of roles, such as development factors and cytokines for cell culture, active enzymes for biochemical assays, carrier proteins for movement of molecules, inhibitors for blocking pathways, and antigens for use with antibodies. Furthermore, we also deal with ready-to-use commercial proteins or peptides that can save a great deal of time and effort. The suppliers we deal with often provide a measure of purity, typically with SDS-PAGE. The recombinant protein search tool offered at ebiohippo gives you the ability to browse through the entire catalog of proteins and peptides from a variety of suppliers. Whereas, our recombinant proteins are purified from E. coli, mammalian cells, and even from cell-free methods.