Humanized Antibody Library

Humanized Antibody Library

Synbio Tech

Traditional humanized antibody library mainly refers to a group of re-expressed antibody which is transformed by gene cloning and DNA recombination technology based on mouse monoclonal antibody. Most of its amino acid sequence was replaced by human source sequence. The affinity and specificity of the parental mouse monoclonal antibody are basically reserved, while its heterology is decreased, which is beneficial to be applied to human body.

Antibody Affinity Maturation Service

Antibody Affinity Maturation Service

Synbio Tech

The binding affinity of an antibody is one of the key parameter to determine the efficacy and dosage of an antibody drug. Currently, the methods to improve the binding affinity of antibodies mainly focus on site-mutation, CDR rearrangement, DNA recombinant, etc. With our unique and effective affinity maturation technology, we can quickly spot the key amino acid site in antibody domain. This, in turn, increases the binding affinity of the antibody, reaching at least nM level.

Antibody Humanization Service

Antibody Humanization Service

Synbio Tech

Immunogenicity or Human Anti-Mouse Antibodies (HAMA) response is what happens when antibodies derived from xenogeneic sources are administered to humans. This can potentially decrease the effectiveness of immune-treatment through rapid clearance of the therapeutic antibody or immune-conjugate. In some cases, the patient may even develop an allergic sensitivity and be at risk of anaphylactic shock upon any future treatment containing these “foreign” antibodies. Due to this potential risk of immunogenicity and HAMA response, the humanization of antibodies from xenogeneic sources to become safer and more reliable humanized antibody drugs is critical.

Single B Cell Antibody Service

Single B Cell Antibody Service

Synbio Tech

The immune system is a very robust “antibody factory” that consistently produces antibodies to fight infections and diseases by recognizing and targeting infected cells or pathogens (antigens). The emergence of single B cell antibody technology has enabled a new strategy for discovery of naturally occurring antibody drugs. The single B cell antibody technology produces higher throughput and higher coverage than the conventional hybridoma or phage display technologies. Most importantly, this technology also avoids the disadvantage of unnaturally paired heavy and light antibody chain displayed by phage.

Single Domain Antibody (sdAb) Service

Single Domain Antibody (sdAb) Service

Synbio Tech

Complex structures and high production costs associated with monoclonal antibodies (mAbs) have limited their achievable clinical applications. For this reason, scientists focusing on antibody drug discovery have shifted their interests toward recombinant antibody fragments.

Custom Monoclonal Antibody Services

Custom Monoclonal Antibody Services

Synbio Tech

Hybridoma technology features the effective usage of innate functions of both immune and cancer cells to generate monoclonal antibodies (mAbs). The resulting hybridoma cell lines are produced to combat specific antigens of interest. Such technology has forged remarkable new approaches toward therapeutic mAb discovery, as well as disease diagnosis and prevention.

Syno® Ab Antibody Design and Production

Syno® Ab Antibody Design and Production

Synbio Tech

In recent years, the proportion of biological drugs in the global pharmaceutical market has been close to 20%. The focus within the global pharmaceutical market has been gradually shifting from small molecule chemical drugs to biomacromolecules (biological drugs). It also appears that the trend is gradually expanding. Biological drugs are a broad class of pharmaceuticals generated by using principles and methods of biology, immunology, physical chemistry and pharmacology, etc. These drugs utilize the composition of various organisms, biological tissues as well as organs. Usually, biological drugs include genetic engineering drugs, antibody engineering drugs, blood products drugs and vaccines etc. Among these, antibody drugs are one of the fastest growing human therapeutic drugs. This increase in research and development has proved to be an effective treatment for many important diseases such as cancer, chronic inflammatory diseases, cardiovascular diseases, immune system diseases and infectious diseases. The antibody research and development also plays an important role in medicine and diagnostics.

T Cell Antibody Sequencing Analysis

T Cell Antibody Sequencing Analysis

Synbio Tech

Over the past few decades, cancer immunotherapy has dramatically altered approaches toward oncology therapy. Although treatment methods such as checkpoint blocking can produce significant clinical responses, the efficacy is uneven between cancer patients and different cancer types. Due to the high heterogeneity and complexity of the tumor microenvironment, the infiltrating lymphocytes need to be analyzed in order to characterize the basic properties of different types on immune cells.

Single B Cell Antibody Sequencing Analysis

Single B Cell Antibody Sequencing Analysis

Synbio Tech

Single B cell antibody technologies offer novel mechanisms for the discovery of naturally occurring antibody drugs. This is accomplished by combining high throughput techniques with low sifting rates and avoiding drawbacks of unnatural pairing from in vitro screening. Synbio Technologies provides single B cell antibody services including the development and preparation of B cell antibodies, B cell antibody sequencing and deep analysis, etc.

Monoclonal Antibody Sequencing

Monoclonal Antibody Sequencing

Synbio Tech

Obtaining the hybridoma monoclonal antibody (mAb) sequence is one of the key requirements of recombinant antibody engineering. Synbio Technologies provides both accurate and efficient monoclonal antibody sequencing services. To accomplish this, we utilize special primer sets and advanced sequencing techniques to help our customers to save on research costs and increase overall efficiency. In doing so, we allow our customers to accomplish their downstream research goals and applications such as antibody humanization, antibody reformatting or cell line transfection.

Immune Repertoire Sequencing

Immune Repertoire Sequencing

Synbio Tech

Immune Repertoire refers to the set of all functionally diversified B cells and T cells in the circulatory system of an individual at any given time. Immune repertoire sequencing (Immuno-Seq) targets at T/B lymphocytes and utilizes the 5’ Rapid Amplification of cDNA Ends (RACE) Technology. The 5’ RACE technology amplifies complementarity-determining regions (CDRs) of B cell receptors (BCRs) or T cell receptors (TCRs). The CDRs are targeted because they control the diversity of the corresponding BCRs and TCRs. Combined with the high-throughput sequencing technology, the Immuno-Seq provides a comprehensive evaluation of the immune system diversity.

ELISA Kits, Antibodies, Proteins, and Reagents for Life Scientists

ebiohippo is a top distributor of the best and most reliable ELISA and Assay kits. We can provide a wide range of kits from well-known suppliers and brands with authentic and validated data. We provide antibodies to help you quickly identify and quantify proteins, and evaluate other necessary data for various applications in life sciences.

Highly Validated Antibodies and Kits Available for Life Sciences Research

Our aim at ebiohippo is to facilitate and accelerate access to health for people everywhere. Through our life science products, we offer solutions for diagnostics and environmental testing as well. We aim to help scientists and engineers solve problems at every stage of their work. The provided lab materials, technologies, and services will help make research, and production, both pharmaceutical and biotech, simpler, faster, and safer. AT ebiohippo, we believe that we can enable scientific discovery by helping customers understand biological functions and diseases. ebiohippo works in partnership with the global scientific community to offer superior products and solutions, both for research, and for manufacturing of chemical and biological drugs.

ebiohippo aims to help you advance in life sciences with high-quality training and support. We can help your highly interdisciplinary bioscience research, from the molecular level, through the cellular and organism levels, as well as spans bacteria, viruses, fungi, animals, and plants. Live science research deals with a broad range of biology, biotechnology, genomics, proteomics, bioinformatics, pharmaceutical and biomedical research, and techniques. We provide you with the products that allow you to identify common principles that operate across the breadth of living systems and investigate solutions to major global challenges. Our products are used in research done with cutting-edge computational and experimental technologies in the life sciences research sector.

ELISA Kits

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A Huge Variety of Elisa Kits from Different Sample Types

Our ELISA kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. All our kits are ready to use and we offer different formats to choose from including quantitative, semi-quantitative, indirect, or competitive. Along with that, our extensive catalog comprises of ELISA kits that can be used to study diverse biochemical pathways and diseases, such as cancer, the insulin pathway, and oxidative stress. Moreover, our kits are only assembled when you place an order to ensure that all of the components will be fresh and to extend the shelf life for our customers.

Assay Kits

ebiohippo offers thousands of Assay kits in multiple formats, including Enzyme activity, inhibitor screening, epigenetic, apoptosis, cell damage, cell proliferation and cytotoxicity, cell signaling, metabolism and other detections.

Easy to Use Kits, Versatile in Functionality and High in Compatibility

Our kits are easy to use yet robust for life science research and bioassays, and are optimized for use on our instruments. They can easily measure enzyme activity, monitor and signal pathway activity, detect and quantify cellular progress such as cell death, cell viability or metabolic reactions. The constituents of every kit are thoroughly tested for their compatibility with each other to give you accurate and authentic results.

Additionally, with our offered assay kits, you’ll be able to study changes in gene expression, and to screen small molecule inhibitors. At ebiohippo, we offer kits that allow researchers to quickly narrow down results by detection target, species reactivity, application or conjugate, and with them, you can obtain highly sensitive, detailed, and accurate results across a wide dynamic range with fast, efficient processing.

Primary Antibodies

At ebiohippo, we offer thousands of monoclonal/polyclonal antibodies and conjugates validated for use in a variety of common applications including WB, FC, IHC, ICC, IF, IP, and more. Our primary antibodies are developed as polyclonal or monoclonal, which use mouse, rat, rabbit, goat, and other animal species as hosts. The primary antibodies provided by us are produced and supplied in various forms, ranging from crude antiserum to antigen-purified preparations. We also offer you a superior antigen recognition system which is combined with the specificity and consistency of a monoclonal, bringing you the highest quality antibodies possible.

Secondary Antibodies

The secondary antibodies provided by ebiohippo perfectly bind to the primary antibody to help them assist in detection, sorting and purifying the target antigens. We offer secondary antibodies that work as staple tools in immunological applications for detecting, quantifying, or purifying target proteins and other molecules. We offer a diverse range of different target species and conjugates that allow a variety of possible combinations and provide flexibility in designing experiments. Along with that, the secondary antibodies we offer often conjugate to other molecules for detection, such as fluorophore, biotin, horseradish peroxidase, or alkaline phosphatase. We also provide you with a general guideline to help you in selecting the appropriate secondary antibody for your experiment.

Proteins and Peptide

With the diverse protein and peptide sources available at ebiohippo, we believe we can fulfill biochemical research needs with ease. The biomolecules we provide can fulfill a diverse range of roles, such as development factors and cytokines for cell culture, active enzymes for biochemical assays, carrier proteins for movement of molecules, inhibitors for blocking pathways, and antigens for use with antibodies. Furthermore, we also deal with ready-to-use commercial proteins or peptides that can save a great deal of time and effort. The suppliers we deal with often provide a measure of purity, typically with SDS-PAGE. The recombinant protein search tool offered at ebiohippo gives you the ability to browse through the entire catalog of proteins and peptides from a variety of suppliers. Whereas, our recombinant proteins are purified from E. coli, mammalian cells, and even from cell-free methods.