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In general, rAAV of various serotypes contain either the ITR sequence (partial sequence of the adeno-associated virus genome) or the WPRE (viral titer enhancer element) of serotype 2. We used the SYBR Green qPCR method. In order to quantify the copy number of recombinant adeno-associated virus genome and determine the titer of recombinant adeno-associated virus, a pair of primers specific to the ITR/WPRE sequence of recombinant adeno-associated virus type 2 were designed to detect the copy number of ITR/WPRE sequence.