ebiohippo is a top distributor of the best and most reliable ELISA and Assay kits. We can provide a wide range of kits from well-known suppliers and brands with authentic and validated data. We provide antibodies to help you quickly identify and quantify proteins, and evaluate other necessary data for various applications in life sciences.
New Cell Membrane HSP70 Antibody
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We are excited to announce the release of a new antibody that is specific for cell membrane embedded heat shock protein 70 (HSP70).
Our anti-HSP70 antibody, clone 1H11 (Catalog No. SMC-249) is unique from other commercially available antibodies in that it can bind to the extracellular region of cell membrane embedded HSP70 protein, allowing researchers to differentiate between intracellular and cell membrane embedded HSP70 across cancer cell types.
Watch our video explaining the importance of cell membrane HSP70 detection:
Cancer & Cell Membrane Embedded HSP70
HSP70 expression is increased due to environmental stressors such as heat, radiation, toxic chemicals, as well as internal stressors such as disease states.
Recent research has found that a portion of HSP70 becomes embedded in the cell membranein tumor cells (1).
The presence of cell membrane embedded HSP70 has been found to increase the stability of cancer cells, thereby protecting tumors from environmental stress (2, 3).
HSP70 Clinical Significance
Patients whose tumor cells have tested positive for cell membrane embedded HSP70 have been shown to have significantly decreased survival rates.
Therefore, in a clinical setting, detection of cell membrane HSP70 could serve as a negative prognostic marker in cancer patients (4).
Research has shown that a side effect of chemotherapy is the upregulation of cell membrane HSP70 on tumor cells (5-8).
This upregulation of HSP70 may be conferring a protective effect on tumor cells, thereby blocking effective reduction of the tumor.
As a result, cancer researchers are exploring the use of HSP70 inhibitors as a potential cancer treatment.
Additionally, cancer researchers are looking to exploit cell membrane embedded HSP70 on tumor cells as a marker for directing tumor cell specific therapies.
Cell Membrane HSP70 Detection
As HSP70 localizes to the cell membrane of cancer cells but not normal cells, it is suggested that HSP70 undergoes a conformational change in the lower pH environment characteristic of cancer cells (7).
Current commercially available antibodies bind to intracellular HSP70, but they are unable to bind to the altered conformation of HSP70 when it is embedded in the cell membrane.
Our new cell membrane HSP70 Antibody is able to bind the altered conformation of HSP70 while embedded in the cell membrane, making it a key tool for cancer research in the life sciences.
It has also shown to bind intracellular HSP70 to a lesser extent when the cell membrane is permeabilized.
Our HSP70 Antibody (SMC-249) has been rigorously validated for use in western blot, flow cytometry, immunocytochemistry/immunofluorescence, and fluorescence-activated cell sorting.
Please visit the HSP70 Antibody (SMC-249) product datasheet for validation figures and further information.
- De Maio, A. (2011) Cell Stress. Chaperones. 16, 235-249.
- Horvath, I. & Vigh, L. (2010) Nature. 463, 436-438.
- Horvath, I., Multhoff, G., Sonnleitner, A., & Vigh, L. (2008) Biochim. Biophys. Acta. 1778, 1653-1664.
- Pfister,K.et al. (2007) Cancer. 110, 926-935.
- Kleinjung, T.et al. (2003) Int. J. Radiat. Oncol. Biol. Phys. 57, 820-826.
- Farkas, B.et al. (2003) Melanoma Res. 13, 147-152.
- Gehrmann, M.et al. (2005) Cell Death. Differ. 12, 38-51.
- Gehrmann, M., Radons, J., Molls, M., & Multhoff, G. (2008) Cell Stress. Chaperones. 13, 1-10
ELISA Kits, Antibodies, Proteins, and Reagents for Life Scientists
Highly Validated Antibodies and Kits Available for Life Sciences Research
Our aim at ebiohippo is to facilitate and accelerate access to health for people everywhere. Through our life science products, we offer solutions for diagnostics and environmental testing as well. We aim to help scientists and engineers solve problems at every stage of their work. The provided lab materials, technologies, and services will help make research, and production, both pharmaceutical and biotech, simpler, faster, and safer. AT ebiohippo, we believe that we can enable scientific discovery by helping customers understand biological functions and diseases. ebiohippo works in partnership with the global scientific community to offer superior products and solutions, both for research, and for manufacturing of chemical and biological drugs.
ebiohippo aims to help you advance in life sciences with high-quality training and support. We can help your highly interdisciplinary bioscience research, from the molecular level, through the cellular and organism levels, as well as spans bacteria, viruses, fungi, animals, and plants. Live science research deals with a broad range of biology, biotechnology, genomics, proteomics, bioinformatics, pharmaceutical and biomedical research, and techniques. We provide you with the products that allow you to identify common principles that operate across the breadth of living systems and investigate solutions to major global challenges. Our products are used in research done with cutting-edge computational and experimental technologies in the life sciences research sector.
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A Huge Variety of Elisa Kits from Different Sample Types
Our ELISA kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. All our kits are ready to use and we offer different formats to choose from including quantitative, semi-quantitative, indirect, or competitive. Along with that, our extensive catalog comprises of ELISA kits that can be used to study diverse biochemical pathways and diseases, such as cancer, the insulin pathway, and oxidative stress. Moreover, our kits are only assembled when you place an order to ensure that all of the components will be fresh and to extend the shelf life for our customers.
ebiohippo offers thousands of Assay kits in multiple formats, including Enzyme activity, inhibitor screening, epigenetic, apoptosis, cell damage, cell proliferation and cytotoxicity, cell signaling, metabolism and other detections.
Easy to Use Kits, Versatile in Functionality and High in Compatibility
Our kits are easy to use yet robust for life science research and bioassays, and are optimized for use on our instruments. They can easily measure enzyme activity, monitor and signal pathway activity, detect and quantify cellular progress such as cell death, cell viability or metabolic reactions. The constituents of every kit are thoroughly tested for their compatibility with each other to give you accurate and authentic results.
Additionally, with our offered assay kits, you’ll be able to study changes in gene expression, and to screen small molecule inhibitors. At ebiohippo, we offer kits that allow researchers to quickly narrow down results by detection target, species reactivity, application or conjugate, and with them, you can obtain highly sensitive, detailed, and accurate results across a wide dynamic range with fast, efficient processing.
At ebiohippo, we offer thousands of monoclonal/polyclonal antibodies and conjugates validated for use in a variety of common applications including WB, FC, IHC, ICC, IF, IP, and more. Our primary antibodies are developed as polyclonal or monoclonal, which use mouse, rat, rabbit, goat, and other animal species as hosts. The primary antibodies provided by us are produced and supplied in various forms, ranging from crude antiserum to antigen-purified preparations. We also offer you a superior antigen recognition system which is combined with the specificity and consistency of a monoclonal, bringing you the highest quality antibodies possible.
The secondary antibodies provided by ebiohippo perfectly bind to the primary antibody to help them assist in detection, sorting and purifying the target antigens. We offer secondary antibodies that work as staple tools in immunological applications for detecting, quantifying, or purifying target proteins and other molecules. We offer a diverse range of different target species and conjugates that allow a variety of possible combinations and provide flexibility in designing experiments. Along with that, the secondary antibodies we offer often conjugate to other molecules for detection, such as fluorophore, biotin, horseradish peroxidase, or alkaline phosphatase. We also provide you with a general guideline to help you in selecting the appropriate secondary antibody for your experiment.
Proteins and Peptide
With the diverse protein and peptide sources available at ebiohippo, we believe we can fulfill biochemical research needs with ease. The biomolecules we provide can fulfill a diverse range of roles, such as development factors and cytokines for cell culture, active enzymes for biochemical assays, carrier proteins for movement of molecules, inhibitors for blocking pathways, and antigens for use with antibodies. Furthermore, we also deal with ready-to-use commercial proteins or peptides that can save a great deal of time and effort. The suppliers we deal with often provide a measure of purity, typically with SDS-PAGE. The recombinant protein search tool offered at ebiohippo gives you the ability to browse through the entire catalog of proteins and peptides from a variety of suppliers. Whereas, our recombinant proteins are purified from E. coli, mammalian cells, and even from cell-free methods.