For Best Results

For Best Results

Reddot Biotech Inc.

Copyright © Reddot Biotech Inc. 2018-2019 All rights reserved

We know that your experiments are important, and we want you to succeed when using Reddot Biotech ELISA kits. That’s why we’ve complied a list of important tips and tricks to help you when you perform your ELISA experiment.

     Storage and Expiration

The shelf life for all our products is 12 months from the time of manufacture. To ensure your product stays viable for the entire shelf life, be sure to store it properly as soon as you receive it.

For our traditional kits, the TMB substrate, Wash Buffer, and Stop Solution should be stored at 4oC, while all other components should be stored at -20oC.

For our new Ready-To-Use kits, all components can be stored at 4oC.

    Reagent and Sample Preparation

Some important tips when collecting and preparing your samples:

-Fresh samples can be kept at 4oC for up to 5 days before use with the assay, but samples collected farther in advance should be stored at -20oC or -80oC (long term storage). It is always best to use fresh samples if possible

-Avoid using any samples that have undergone hemolysis

-Be sure to bring samples to room temperature before beginning the assay

-Ensure that the samples used with the kit fall within the detection range, diluting samples if necessary. If you are unsure of the sample concentration, perform a preliminary experiment to determine the best dilution ratio for your samples. Samples should be diluted using 0.01mol/L PBS (pH 7.0-7.2)

-If you are using a chemical lysis buffer to prepare your samples and you are unsure if it will interfere with the kit, please contact us before beginning your experiment

-Cell culture supernatant samples will not always be detected by our kits due to factors such as cell viability and cell volume at the time of collection

Some important tips when preparing the reagents included in the kit:

-Make sure all components are at room temperature before beginning the experiment

-Mix all reagents before beginning the experiment 

-Do not let the prepared standard sit for extended periods of time before use, it is best to prepare the standard within 15 minutes of performing the assay

-We recommend performing the serial dilution in clean microcentrifuge tubes, NOT directly in the wells of the plate

-If you are using a Traditional kit, note that detection reagents A and B are sticky solutions and need to be pipetted slowly

-Be gentle when preparing the standard solutions and detection solutions A and B. Avoid foaming when mixing these solutions for best results

-Ensure any pipettes that are being used have been properly calibrated to prevent volume and concentration errors

-Crystals in the Wash Solution Concentrate are normal after periods of storage. Be sure to bring the solution to room temperate and dissolve all crystals by mixing gently before use

-Be sure to start with clean supplies, including any beakers, tubes, and tips, to ensure there is no contamination

     Assay Procedure

Some important tips to keep in mind when preforming the assay procedure:

-Calculate the amount of wells you will need to complete your experiment, and store any extra strips at -20oC

-Always be careful when adding solutions to the wells and avoid touching the well walls to ensure accurate results

-Ensure the time for each pipetting step is the same for accurate time results (ie, avoid taking breaks in the middle of the plate)

-To avoid contamination, do not let the wells sit uncovered for extended periods of time, and always ensure plate sealers are properly applied during incubation steps

-Once the assay has been started, do not let the wells dry out for the entirety of the experiment

-Washing is one of the most important parts of ELISA assay procedure. For best results, have one experimenter perform the entire assay to ensure the procedure is consistent for all washing steps. Ensure that all liquid in the wells is removed during each wash step

-Monitor the reaction time once the TMB substrate has been added (we recommend once every 10 min). If the colour changes quickly, add the stop solution whenever necessary to ensure accurate results

-Be sure to protect the TMB substrate from light contamination when using it

-Keep the temperature and humidity constant while preforming your assay to ensure environmental changes do not affect your results

    Results Calculations

When calculating your results, construct your standard curve first, then plot your sample OD values to determine their concentration.

Some important tips when calculating the results of your assay:

-We recommend using curve plotting software for the most accurate results. We do not recommend only using Microsoft Excel to calculate your results

-Remember to plot the OD values on the x-axis and the concentration values on the y-axis

-For competitive inhibition kits, note that the results will be reverse proportional to the concentration in your samples

-Make sure to multiply your results by the dilution factor if your samples were diluted

If you have any questions or comments about this article or your ELISA kit, feel free to contact us here or send us an email at!

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ELISA Kits, Antibodies, Proteins, and Reagents for Life Scientists

ebiohippo is a top distributor of the best and most reliable ELISA and Assay kits. We can provide a wide range of kits from well-known suppliers and brands with authentic and validated data. We provide antibodies to help you quickly identify and quantify proteins, and evaluate other necessary data for various applications in life sciences.

Highly Validated Antibodies and Kits Available for Life Sciences Research

Our aim at ebiohippo is to facilitate and accelerate access to health for people everywhere. Through our life science products, we offer solutions for diagnostics and environmental testing as well. We aim to help scientists and engineers solve problems at every stage of their work. The provided lab materials, technologies, and services will help make research, and production, both pharmaceutical and biotech, simpler, faster, and safer. AT ebiohippo, we believe that we can enable scientific discovery by helping customers understand biological functions and diseases. ebiohippo works in partnership with the global scientific community to offer superior products and solutions, both for research, and for manufacturing of chemical and biological drugs.

ebiohippo aims to help you advance in life sciences with high-quality training and support. We can help your highly interdisciplinary bioscience research, from the molecular level, through the cellular and organism levels, as well as spans bacteria, viruses, fungi, animals, and plants. Live science research deals with a broad range of biology, biotechnology, genomics, proteomics, bioinformatics, pharmaceutical and biomedical research, and techniques. We provide you with the products that allow you to identify common principles that operate across the breadth of living systems and investigate solutions to major global challenges. Our products are used in research done with cutting-edge computational and experimental technologies in the life sciences research sector.


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A Huge Variety of Elisa Kits from Different Sample Types

Our ELISA kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates. All our kits are ready to use and we offer different formats to choose from including quantitative, semi-quantitative, indirect, or competitive. Along with that, our extensive catalog comprises of ELISA kits that can be used to study diverse biochemical pathways and diseases, such as cancer, the insulin pathway, and oxidative stress. Moreover, our kits are only assembled when you place an order to ensure that all of the components will be fresh and to extend the shelf life for our customers.

Assay Kits

ebiohippo offers thousands of Assay kits in multiple formats, including Enzyme activity, inhibitor screening, epigenetic, apoptosis, cell damage, cell proliferation and cytotoxicity, cell signaling, metabolism and other detections.

Easy to Use Kits, Versatile in Functionality and High in Compatibility

Our kits are easy to use yet robust for life science research and bioassays, and are optimized for use on our instruments. They can easily measure enzyme activity, monitor and signal pathway activity, detect and quantify cellular progress such as cell death, cell viability or metabolic reactions. The constituents of every kit are thoroughly tested for their compatibility with each other to give you accurate and authentic results.

Additionally, with our offered assay kits, you’ll be able to study changes in gene expression, and to screen small molecule inhibitors. At ebiohippo, we offer kits that allow researchers to quickly narrow down results by detection target, species reactivity, application or conjugate, and with them, you can obtain highly sensitive, detailed, and accurate results across a wide dynamic range with fast, efficient processing.

Primary Antibodies

At ebiohippo, we offer thousands of monoclonal/polyclonal antibodies and conjugates validated for use in a variety of common applications including WB, FC, IHC, ICC, IF, IP, and more. Our primary antibodies are developed as polyclonal or monoclonal, which use mouse, rat, rabbit, goat, and other animal species as hosts. The primary antibodies provided by us are produced and supplied in various forms, ranging from crude antiserum to antigen-purified preparations. We also offer you a superior antigen recognition system which is combined with the specificity and consistency of a monoclonal, bringing you the highest quality antibodies possible.

Secondary Antibodies

The secondary antibodies provided by ebiohippo perfectly bind to the primary antibody to help them assist in detection, sorting and purifying the target antigens. We offer secondary antibodies that work as staple tools in immunological applications for detecting, quantifying, or purifying target proteins and other molecules. We offer a diverse range of different target species and conjugates that allow a variety of possible combinations and provide flexibility in designing experiments. Along with that, the secondary antibodies we offer often conjugate to other molecules for detection, such as fluorophore, biotin, horseradish peroxidase, or alkaline phosphatase. We also provide you with a general guideline to help you in selecting the appropriate secondary antibody for your experiment.

Proteins and Peptide

With the diverse protein and peptide sources available at ebiohippo, we believe we can fulfill biochemical research needs with ease. The biomolecules we provide can fulfill a diverse range of roles, such as development factors and cytokines for cell culture, active enzymes for biochemical assays, carrier proteins for movement of molecules, inhibitors for blocking pathways, and antigens for use with antibodies. Furthermore, we also deal with ready-to-use commercial proteins or peptides that can save a great deal of time and effort. The suppliers we deal with often provide a measure of purity, typically with SDS-PAGE. The recombinant protein search tool offered at ebiohippo gives you the ability to browse through the entire catalog of proteins and peptides from a variety of suppliers. Whereas, our recombinant proteins are purified from E. coli, mammalian cells, and even from cell-free methods.