Human IL-27(Interleukin-27 subunit alpha) ELISA Kit

Mfr.No.

EH0193

Alternative Names

IL-27 ELISA Kit, IL-27 subunit alpha ELISA Kit, IL-27-A ELISA Kit, IL27-A ELISA Kit

Reactivity

Human

Detection Method

Double Antibody

Detection Range

0.156-10ng/ml

Sample Type

Cell lysate, Tissue lysate, Other liquid samples, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Recovery

Matrices listed below were spiked with certain level of IL-27 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-27 in samples.

Matrix	Recovery range(%)	Average(%)
serum(n=5)	86-96	90
EDTA plasma(n=5)	89-98	93
heparin plasma(n=5)	85-100	93

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-27 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8
serum(n=5)	87-104%	91-101%	92-99%
EDTA plasma(n=5)	83-92%	90-98%	82-96%
heparin plasma(n=5)	80-100%	80-95%	91-97%

Sensitivity

< 0.094ng/ml

Components

Microplate,Lyophilized Standard,buffer,SABC,Label antibody,SABC,TMB,stop solution

Background

IL-27(IL-27 subunit alpha)/IL-27-A/IL27-A

Notes

For Research Use Only

UniProt ID	Q8NEV9
Antigen	IL-27

Applications	ELISA
Applications Notes	IL-27 ELISA Kit allows for the in vitro quantitative determination of IL-27 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision	Intra-Assay: CV<8% Inter-Assay: CV<10%

Storage	2-8 °C for 6 months

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SKU	Supplier No.	Size	Your price	Quantity
BHE10806018	EH0193	96T	$350		Add to Cart

Human IL-27(Interleukin-27 subunit alpha) ELISA Kit

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